# Real time PCR Calculator

Cost Calculator View Referral View
Cost calculator is not optimized to function on IE7 or IE8. For optimal performance, please use Chrome, IE9+, Firefox or Safari.
Currency
Real Time PCR Price Calculator
Need Help

### What type of experiment are you performing?

Application
Gene Expression miRNA Genotyping Copy Number Variation Mutation Detection Pharmacogenomics Custom Plating
Species
Human Mouse Rat
Consumable format
All Tube TaqMan® Array Plate - Fast TaqMan® Array Plate - Standard TaqMan® Array Card OpenArray Plates QuantStudio 3D Chip
Select Taqman Assay Type
(applicable only for QS3D)

### Experimental Information

# of targets
# Reference targets ( N/A for Digital PCR)
# of samples
# of replicates
Choose a format ( Applicable only when you use your own microtiter plates)
96-well
(20 uL rxn) 384-well
(10 uL rxn) Tube
Include IPC
Reference Assay
Chemistry
Fast Standard
Preamplification required

### Instruments

Technology
Both Real-time PCR Digital PCR
Instrument
Any Instrument Show products options for all instruments.
QuantStudio 12K Flex
7900 HT
7500 Fast
7500
StepOne Plus
ViiA 7
NT Cycler
7300
7000
QuantStudio 6
QuantStudio 7
QuantStudio 5
QuantStudio 3
QS3D
Other Third party instruments. Includes plates and single tube products.
Calculate Prices

### Experimental Information

How many samples will you be running Calculations are based on 365 days per year, 52.177 week per year and 12 months per year.
Calculate consumables and reagents for days
Generate quote for : Consumables only Instruments and Consumables?
DNA Extraction
How many MagMax system do you need for DNA extraction Enter 0 if you don't need DNA extraction solution.
Estimate # of instruments

Max sample throughput per day |
Max sample throughput per year |
% use of instrument |
Recommended # instruments |

SNP Analysis

Which instrument do you want to use for SNP analysis To generate a quote for consumable and reagent only, select instrument and format and enter 0 for number of instrument needed. |
Which format do you need? |
How many SNPs per sample are you processing? |
Number of replicates to be run? |
How many instruments or blocks do you need? |
How many Accufil instruments do you need Recommend 1 instrument per QS OpenArray instrument. |
Select type of assay you would like to include: |

Estimate # of instruments

Max sample throughput per day |
Max sample throughput per year |
% use of instrument |
Recommended # instruments |

Copy Number Analysis

On average, how many copy number variants will you analyze per sample To generate a quote for consumable and reagents only, select instrument and format and enter 0 for number of block needed. |
Which instrument do you want to use for CNV? |
Which format do you want to use? |
How many instruments or blocks do you need? |
Select type of assay you would like to include: |

Estimate # of instruments

Max sample throughput per day |
Max sample throughput per year |

% use of instrument |
Recommended # instruments |

Installation and Training

Do you need content IQ/OQ Content IQ/OQ consists of validation of the specification. | Yes No
Do you need computer system validation Computer system validation consists of… | Yes No
Which type of instrument installation do you need We recommend to select XXX because includes X, Y and Z. |
Which type of maintenance contract do you want? |
Which additional training do you want Trainings provided with instrument are jfdlkalfjdlak. Select if you need additional training. |

Generate Quote
Your referral has been sent successfully
Do you want to make another referral?

### Refer Project Details to LSG Rep

Provide informtion below to refer customer to an LSG rep

### Customer Details

First Name
Last Name
Email
Institution
Please note many universities start with the abbreviated "Univ". For some accounts, you will also need to test different abbreviations to find the appropriate match.

### Referred By

Name
Type of Rep
Comments
Email
Send Referral
Consumable Types
OpenArray Plates
Assay Types
Gene Expression miRNA SNP CNV Cast PCR
Experimental Information
# of Inv Assays
# of samples
# of replicates

Does the project include any custom assays? |
PREAMP Rxn Size: | 5uL 10uL 20uL 25uL 40uL 50uL
RT POOLS | Megaplex Custom RT

View Estimated Pricing
Compare experiment options in a few easy steps
1Fill experiments parameters.
2 Get recommendations and costs for various options.

- * Assay in tubes(Inventoried, MTO or Custom)
- * TaqMan® Array Plates (Fixed and Custom)
- * TaqMan® Array Cards (Fixed and Custom)
- * TaqMan® OpenArray Plates (Fixed and Custom)
- * SYBR Green Primers

3Adjust pricing with desired discount levels.
< Back to Results
You must have Flash 10 installed to download this file. E-mail Product List
Total cost of experiment
Price per reaction
Per sample
Total hours

targets reference targets samples replicates
Assays samples replicates
E-mail sent successfully
X

To: E-mail addresses separated by comma |
From: |
Subject: |
Msg: |

SendMail

## Product List

Product | #rxns | Part number | Quantity | Unit Price | Vol Disc | Total Discount | Price per rxn | Price per sample | Net Price
--- | --- | --- | --- | --- | --- | --- | --- | --- | ---

Optimization Cost
Total Cost
Optimization Costs(for Sybr)

Reordering of primers |
Additional master mix (ml) |
Additional master mix cost |
Additional labor |
Total optimization cost |

Quote Delivery Information

Delivery Method |
Send to customer by |
Send to sales rep by |
Account number |
Original quote number,if applicable |
Attention to |
Institution |
Department |
Building |
Address |
City, State |
Zipcode |
Phone |
Extention |
E-mail |
Fax |

E-mail sent successfully
X

To: E-mail addresses separated by comma |
From: |
Subject: |
Msg: |

SendMail
Quote Summary

DNA Extraction | $10,000
SNP Analysis | $10,000
CNV Analysis | $10,000
Service | $10,000
Training | $10,000
Total | $110,000
Cost per sample | $30

Experiment costs

Modify Experiment
Change Experiment
Experiment Information
# of targets
# of inv assays
# of custom assays
Reference targets
# of samples
# of replicates
Preamp needed
Fast Standard
96-well 384-well Tube
Discounts
Assay
Master Mix
Plates/Cards
Accessories
OpenArray
RT/PreAmp
Hide SYBR Information
Price for pair of primers
Percent of SYBR primers redesigned
Hourly labour rate for optimization
Number of hours required for design and optimization(per primer pair)

### Cost Summary

All | Fixed Content | Flexible Content
Tubes | N/A |
Plates |  |
Cards |  |
OpenArray |  |
QS 3D Chips |  |

Lowest Cost option
Fastest workflow

Modify Parameters
Sort by: Price Time
Need Help
Fixed Panels (plate/array) are not shown. Show Fixed Content:
Some TaqMan Array Plates, Cards or OpenArray product options were not included because the number of targets specified is higher than what is supported by those products.
Here are the maximum number of tagrets supported by plates, cards and arrays:
Plates - Custom: 96
Plates - Fixed Panels: 96
Cards - Custom: 384
Cards - Fixed Panels: 384
OpenArray - Custom: 112
OpenArray - Fixed Panels: 768
Graph View
List View
Inputs
Quote Summary

DNA Extraction |
SNP Analysis |
CNV Analysis |
Service |
Training |
Total |
Cost per sample |

Export Products
Detailed Quote
DNA Extraction
SNP Analysis
CNV Analysis
Service Plan and Training
Show All Products
Show Recommended Products
Export Products
Sample
Volume discount:
Total discount:
Submit Close
Close

#### Input Page

Experiment inputs vary based on application type selection. Currently the following types of experiments are supported:

- Gene Expression
- MicroRNA
- SNP Genotyping
- Digital PCR
- Copy Number Variation
- Mutation Detection

#### Gene Expression

TaqMan® Gene Expression Assays are primer/probe sets for quantitative gene expression analysis. Choose from over 1.3 million predesigned primer/probe sets or design your own Custom TaqMan® Gene Expression Assays.

### Experiment Information

Input | Description | How this information is used
--- | --- | ---
Number of samples | Number of samples in the experiment | Number of samples is used to calculate the total number of reactions.
Number of targets | Number of genes in the experiment | Number of targets is used to calculate the total number of reactions.
Number of replicates | Number of technical replicates | Number of replicates is used to calculate the total number of reactions. Total number of reactions = Number of targets * Number of Samples * Number of replicates
Species | Choose one of Human, Mouse or Rat | Species information is used to recommend correct product options and part numbers.
Plate Format | This option applies only to tube-based assays. 96-well: Select this option if experiment will be run using 96-well plates. 384-well: Select this option if experiment will be run using 384 well plates | This information is used to calculate the amount of assay and master mix needed. If 96-well option is selected, 20uL reaction volume is used. For 384-well, 10uL reaction volume is used.
Chemistry | Choose from Fast or Standard chemistry. | When Fast option is selected, Fast part numbers will be recommended.

Instrument Type

Options: Any instrument, QuantStudio™ 12k Flex,7900 HT,7500 Fast, 7500, StepOne-Plus™,ViiA™ 7, 7300, NT Cycler and 7000.

Assay formats displayed depend on the block types available on the selected instrument.

For example, if StepOne Plus instrument is selected, only plates and single tube assays are displayed. If QuantStudio™ is selected, tubes, plates, cards and OpenArray® options are included.

Default value is ‘Any Instrument’. In this case all product options are displayed.

Currency | Select the currency in which costs are displayed. |

#### SNP Genotyping

TaqMan® SNP genotyping assays exploit the precision of TaqMan® probe-based chemistry for genotyping studies. Over 4.5 million human and mouse TaqMan® SNP Genotyping Assays and 2700 DME (drug metabolism enzyme) assays are available, or design your own Custom TaqMan® SNP Genotyping Assays.

### Experiment Information

Input | Description | How this information is used
--- | --- | ---
Number of targets | Number of SNPs in the experiment | Number of SNPs used to calculate the total number of reactions.
Number of samples | Number of samples in the experiment | Number of samples is used to calculate the total number of reactions.
Number of replicates | Number of technical replicates | Number of replicates is used to calculate the total number of reactions. Total number of reactions = Number of targets * Number of Samples * Number of replicates.
Species | Choice of Human and Mouse | Species information is used to recommend the correct product options and part numbers
Plate Format | This option applies only to tube-based assays. 96-well: Select this option if experiment will be run using 96-well plates 384-well: Select this option if experiment will be run using 384 well plates | This information is used to calculate the amount of assay and master mix needed. If 96-well option is selected, 25uL reaction volume is used. For 384-well, 5uL reaction volume is used.

Instrument type

Options: Any instrument, QuantStudio™ 12k Flex,7900 HT,7500 Fast, 7500, StepOne-Plus™,ViiA™ 7, 7300, NT Cycler and 7000.

Assay formats displayed depend on the block types available on the selected instrument.

For example, if StepOnePlus instrument is selected, only plates and single tube assays are displayed. If QuantStudio is selected, tubes, plates, cards and OpenArray options are included.

Default value is ‘Any Instrument’. In this case all product options are displayed.

Currency | Select the currency, in which costs are displayed. |

#### MicroRNA

TaqMan® MicroRNA Assays employ a target-specific stem-loop reverse transcription primer to interrogate short length mature miRNAs. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan® Assay–based real-time PCR. TaqMan® MicroRNA Assay content is aligned to miRBase v.19; Custom TaqMan® Small RNA Assays are available for miRNAs not included in miRBase and for any other Small RNAs.

### Experiment Information

Input Page | Description | How this information is used
--- | --- | ---
Number of Targets | Number of microRNAs in the experiment | Number of targets is used to calculate the total number of reactions.
Number of Samples | Number of samples in the experiment | Number of samples is used to calculate the total number of reactions.
Number of Replicates | Number of technical replicates | Number of replicates is used to calculate the total number of reactions. Total number of reactions = Number of targets * Number of Samples * Number of replicates.
Species selection | Human, Mouse, Rat | Species information is used to recommend the correct product options and part numbers.
Plate Format | This option applies only to tube-based assays. 96-well: Select this option if experiment will be run using 96-well plates. 384-well: Select this option if experiment will be run using 384 well plates. | This information is used to calculate the amount of assay and master mix needed. If 96-well option is selected, 20uL reaction volume is used. For 384-well, 10uL reaction volume is used.

Instrument type

Options: Any instrument, QuantStudio™ 12k Flex,7900 HT,7500 Fast, 7500, StepOnePlus™,ViiA™ 7, 7300, NT Cycler and 7000.

Assay formats displayed depend on the block types available on the selected instrument.

For example, if StepOnePlus instrument is selected, only plates and single tube assays are displayed. If QuantStudio™ is selected, tubes, plates, cards and OpenArray® options are included.

Default value is ‘Any Instrument’. In this case all product options are displayed.

Currency | Select the currency, in which costs are displayed. |
Pre-Amp needed Checkbox | When checked, reagents needed for pre-amplification are included |

#### Digital PCR

Digital PCR applications such as accurate, traceable quantification of viral load, sensitive detection of mutant sequences against backgrounds of somatic wild type DN A, and GMO quality control, can be performed using the Quantstudio® 12k Flex Real-time PCR system and TaqMan® OpenArray® Digital PCR Plates.

### Experiment Information

Input | Description | How this information is used
--- | --- | ---
Number of Targets | Number of genes in the experiment | Number of targets is used to calculate the total number of reactions.
Number of Samples | Number of samples in the experiment | Number of samples is used to calculate the total number of reactions.
Number of Subarrays | Number of subarrays per sample | Number of subarrays is used to calculate the total number of open arrays needed.
Instrument Selection | Digital PCR can be done with two instruments only namely QuantStudio™ 12k Flex and NT Cycler. |
Currency | Select the currency, in which costs are displayed. |

#### Copy Number Variation

TaqMan® Copy Number Assays use TaqMan® probe-based chemistry for copy number variation studies. Over 1.6 million assays are available, predesigned with human, genome-wide coverage, and mouse and marker/reporter assays. Custom and Custom Plus TaqMan® Copy Number Assay options are ideal when a predesigned assay for a target is not available.

### Experiment Information

Input | Description | How this information is used
--- | --- | ---
Number of Targets | Number of CNVs in the experiment | Number of targets is used to calculate the total number of reactions.
Number of Samples | Number of samples in the experiment | Number of samples is used to calculate the total number of reactions.
Number of Replicates | Number of technical replicates | Number of targets is used to calculate the total number of reactions. Total number of reactions = Number of targets * Number of Samples * Number of replicates
Reference Assay Selection | Select RNase P or TERT as the reference assay | This information is used to recommend the qty of reference assay needed to carry out the experiment.
Currency | Select the currency, in which costs are displayed. |

#### Mutation Detection

TaqMan® Mutation Detection Assays employ Competitive Allele-Specific TaqMan® PCR (castPCR™) technology to detect and quantitate rare sequences in a background of common sequence by specifically blocking amplification of all but the rare-sequence variants.

### Experiment Information

Input | Description | How this information is used
--- | --- | ---
Number of Targets | Number of mutations in the experiment. | Number of targets is used to calculate the total number of reactions.
Number of Samples | Number of samples in the experiment. | Number of samples is used to calculate the total number of reactions.
Number of Replicates | Number of technical replicates. | Number of targets is used to calculate the total number of reactions. Total number of reactions = Number of targets * Number of Samples * Number of replicates
# of Reference Targets | Number of reference targets in the experiment. | This is used to calculate the number of reactions for reference targets.
Currency | Select the currency, in which costs are displayed. |
Include IPC | This checkbox allows adding IPC (internal positive control) reagents | When selected, IPC (internal positive control) reagent kit is added to the list of products.

Top
Close

#### Product Options

This page displays all product options for the application and instrument selected on input page.

For a given product type, the tool evaluates all package sizes and recommends the most optimal size based on cost.

The list is sorted by price from the lowest to the highest.

Field | Description
--- | ---
Cost Summary | Cost Summary table shows a price summary of all product options grouped by assay format (Tubes, Plates, Cards and OpenArray®). The table also acts as a filtering mechanism to view all products of a specific format. For example, clicking Tubes filters the products list and only shows tube-based assays.
Lowest Cost Option | Lowest cost product option for the specified experiment. Clicking on the product name shows details of the product option.
Fastest Workflow Option | Product that offers fastest time to results (or the least amount of time spent in the workflow). Clicking on the product name shows details of the product option.
Sort by Price/time | Allows sorting of the product list in ascending order of price or workflow time.
Graph view | This is a bar graph of product prices or workflow time by assay format.
Discounts | Enables the application of discounts to products by product type. For example, changing Assay discount will apply the specified discount to all assay products. All prices are updated in real-time with the specified discounts.
Minimum quantity warning | If the number of units calculated by the tool for the specified experiment inputs is less than the minimum purchase quantity for the product, a warning message is displayed with a yellow triangle icon. For example, if the user specifies 24 targets and 3 samples for a gene expression experiment, only one TaqMan® Array Plate is required to meet the experiment needs, but the tool recommends 6 units, which is the minimum purchase quantity.
Total price | This is the total price for all products included in the product option. Products included vary by product option and format, but at a minimum, assays and master mix are included.
Price per reaction | Price per reaction is calculated by dividing the total price by the total number of reactions derived from number of targets, samples and replicates specified in the experiment inputs. This calculation does not take into account utilizing all usable quantity purchased.
Price per sample | Price per sample is calculated by dividing the total price by number of samples specified in experiment inputs. This calculation does not take into account utilizing all usable quantity purchased.

Number of units

Number of units is calculated by considering the following factors:

- Total number of reactions

- Optimal package size

- Optimal product format (for example, the right plate format based on the number of genes for TaqMan® Array Plates)

Top

### Applications

Gene Expression

- Overview
- Customer Segmentation
- Talking to customers
- Workflow and Products.

Genotyping

- Overview
- Customer Segmentation
- Talking to customers
- Workflow and Products.

Micro RNA

- Overview
- Customer Segmentation
- Talking to customers
- Workflow and Products.

### Key Products

TaqMan Gene Expression Assays
TaqMan Array Cards and Plates
TaqMan OpenArray Plates

### Competitors

Bio-rad
Roche
Qiagen
IDT DNA